Steroid cross reactivity chart7/31/2023 ![]() ![]() A volume >3.0 mL is generally accepted as the beginning of puberty with increasing testicular secretion of TE. Pubertal development in boys starts with an enlargement in testicular size which is reliably measurable with Prader’s size-standardized orchidometer. Gonadarche is induced by the pulsatile gonadotropin-releasing hormone (GnRH) secretion followed by rising luteinizing hormone (LH) and follicle-stimulating hormone (FSH) leading to an increase in testicular volume (TV). The phenotypic effect of adrenarche is the development of axillary and pubic hair (PH). Adrenarche is the result of an increased production of the adrenal steroids androstenedione (AE), dehydroepiandrosterone (DHEA) and its sulfate ester DHEA-sulfate (DHEAS) which, for example, act as precursors for testosterone (TE) in peripheral tissues. Male pubertal development comprises both adrenarche and gonadarche. The consideration of further salivary sex steroids did not improve diagnostic accuracy. Salivary TE permits a good non-invasive characterization of pubertal maturation stages. Inclusion of further salivary steroids did not improve analysis results. Binomial logistic regression analysis revealed significant correlations between salivary TE and pubertal maturation during the development phases of prepuberty-puberty and puberty-postpuberty. Correlations between salivary and serum concentrations of steroids were also statistically significant (p<0.001). Using Spearman rank correlation, salivary steroids significantly correlated (p<0.001) with pubertal development: TE (TV r=0.74 and PH stages r=0.72), DHEA (r=0.58 and 0.62), AE (r=0.38 and 0.45) and 17-OHP (r=0.42 and 0.43). Validation of saliva-based ELISA tests included data related to inter- and intra-assay coefficients of variation (CVs), recovery and linearity. Four different sex steroids (testosterone, dehydroepiandrosterone /dehydroepiandrosterone-sulfate, androstenedione (AE), 17-hydroxyprogesterone ) were measured in saliva by enzyme-linked immunosorbent assay (ELISA) and as serum total steroids by different assays (radioimmunoassay, chemiluminescence immunoassay, electrochemiluminescence immunoassay ). Pubic hair (PH) stages were stratified by Tanner and testicular volume (TV) by means of the Prader orchidometer and assigned to the prepubertal, pubertal and postpubertal development phase. The investigation included 165 normal boys (mean age 12.7☒.8 years, mean body mass index 19.6±4.2 kg/m 2). However, relationships between various sex hormones and pubertal development stages have not been sufficiently studied. During pubertal development in healthy boys, increased levels of different sex steroids occur which are responsible for sexual maturation and physical changes.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |